Single Cell Dispensing

Single cell dispensation involves the precise placement of isolated single cells or groups of cells into microwell plates or nano-well arrays using a dispenser. This process requires high accuracy to ensure that each cell is correctly positioned without contamination or loss of viability. 
Maintaining cellular viability and integrity during the process, achieving high precision and accuracy in cell placement, and preventing contamination become challenging tasks during high-throughput experimentation. The ARRALYZE CellShepherd® single-cell dispenser generates droplets on demand with live control of dispensing, ensuring integrity during single-cell sequencing.

Technology of
Single Cell Dispensing

 

 

The ARRALYZE CellShepherd® offers innovation, ease of use, and flexibility to meet your research needs. Designed for fast, easy, and gentle single cell dispensing, the CellShepherd® single cell dispenser operates within a sterile interior, ensuring flexibility and effortless use. 

 

ARRALYZE® cell analysis platform features defect-free small structures and high aspect ratios in standard glass types. Built around these glass arrays are a cell dispenser, an inverted microscope, and a cell extraction unit, making CellShepherd® an exceptional single cell analysis platform.

 

Structures are created using laser-induced deep etching (LIDE) technology. They are made up of defect-free glass arrays with a high aspect ratio. They can accommodate anywhere from a few to hundreds of cells in a very small space

 

Key Features of
Single Cell Dispensing

 

These are the advantages of using CellShepherd for single cell dispensing

 

  • Achieve high viability after dispensing cells in single cell analysis through a gentle dispensing method
  • Achieve precise dispensing of cell suspensions with excellent viability
  • Live control of dispensing
  • Automate the dispensing of single cells, cell populations, and particles
  • Dispense precisely into glass nanowell arrays
  • High-precision calibration of the drop volume
  • High precision and programmable dispensing routine
  • Immediately imaging and recording wells
  • The sterile interior ensures contamination-free dispensing
  • Conduct time-resolved measurements with ease on dispensed single cells

 

Applications of Single Cell Dispensing

  • Cell line development
  • Monitoring cell toxicity in real-time
  • Cell and gene therapy
  • Understanding tumor heterogeneity in cancer cells
  • Investigating the immune response at single cell level
  • Understanding stem cell differentiation
  • Gaining insights into embryonic development
  • Screening drugs and testing their effects on individual cells

 

  • Monoclonal antibodies
  • Single cell sorting
  • Isolation of stem cells for regenerative medicine
  • Recombinant protein production
  • Genomics & cDNA library generation
  • Vaccine production
  • Single cell genomics

Webinars and Publications

Webinar

Advancing Single-Cell Analysis with Dispensing and Isolation

Publication

Lab on a Chip

Blog

NK Killing Assays

Logo: ARRALYZE  LPKF Laser & Elektronics
Logo: ARRALYZE  LPKF Laser & Elektronics