Discovering Cellular Diversity
with Single Cell Analysis

Single cell analysis uncovers unique functional characteristics by examining individual cells. Single cell isolation enabling downstream techniques like sequencing allows researchers to gain an in-depth understanding of cellular heterogeneity.

The study of single cell analysis is challenging. The challenges include ensuring cell viability during single cell isolation, identification of cells of interest during the experiments, high-throughput experiments, and maintaining genomic integrity during single cell sequencing.

Technology of Single Cell Analysis

ARRALYZE CellShepherd^® revolutionizes the process of single cell analysis through a large number of parallel experiments. ARRALYZE works with nanowells that are ideal for culturing just a few or even a single cell in just the right amount of medium.

With our glass nanowells, single cells are immobilized in a small, confined area that can be continuously imaged. AI-driven algorithms identify and track single cells, while the inert material and controlled environment allow you to observe the cells in their true physiological state. This ensures that single cell analysis is precise and accurate, facilitating the isolation of single cells for subsequent analysis.

Time-lapse video of bovine dedifferentiated fat cell becoming adherent, demonstrating the effectiveness of automated imaging for time-resolved single-cell assays.

The CellShepherd's AI-driven algorithm identifies and tracks individual cells throughout the experiment, enabling precise monitoring of cell behavior, growth, and responses.

The ARRALYZE CellShepherd^® nanowell glass microarray transforms single cell imaging with its advanced laser-induced deep etching (LIDE) technology. Our innovative, proprietary LIDE manufacturing process is used to create a glass microarray that maintains a high aspect ratio, while the structure remains free of stresses or microdefects. Glass-based microarrays are ideal for single cell technology due to their inert environment and optimal properties for various techniques such as single cell imaging, single cell sequencing, single cell isolation, single cell assay, single cell profiling, and single cell culture.

The ARRALYZE CellShepherd^® nano-well glass microarray is characterized by its ability to create deep or shallow wells that effectively confine cells, which is a great advantage for single cell analysis. The ability to customize the shapes and sizes of wells in microfluidic glassware increases its versatility and allows it to be used for high-throughput experiments.

Left: Flat Bottom Well or F-Well; Right: Dimpled Bottom Well or W-Well.

Use our cutting-edge technology for single cell analysis to explore the benefits of live single cell monitoring
and drive advances in biomedical research.

How does it work?

Key Features of
Single Cell Analysis

Single cell assays provide a more nuanced view of cell populations compared to traditional bulk experiments that only provide averaged data. Live cell imaging can be used to generate single cell analysis data. ARRALYZE CellShepherd^® has the following key features:

Single cell dispensing ensures precise deposition, high immobilization efficiency, and easy recovery for single cell analysis
Perform single cell live experiments on the specialized nanowell glass array with precise spatial confinement of cells
Perform high throughput single cell analysis on a small footprint with a high aspect ratio that produces thousands of wells
A gentle dispensing method enables high viability after dispensing cells in single cell analysis
Gain insight at every stage of biological experiments in nanowells during single cell analysis
Array layouts optimized for single cell screening allow scientists to adjust the size and shape of wells for single cell isolation depending on cell type and experiment