Overcome the Challenges of Cell Line Development
Streamline your cell line development workflow with unmatched speed and quality
The development of stable cell lines plays a crucial role in drug screening, gene studies and the production of biologics such as recombinant proteins and monoclonal antibodies. The protocol for stable cell line development involves the transfection of mammalian cell lines like CHO or HEK293 cells with specific plasmids to initiate cell line formation.
After transfection, scientists identify the transfected cells and perform screening to identify the high expression clones and then validate the cell lines and/or the proteins they produce. Despite technological advances, the process of developing cell lines is fraught with challenges:
- Inefficient single cell isolation
- Sorting is typically harsh (mechanical forces and shear stress)
- Poor growth of single cells in microtiter plates
- Time-consuming manual cell screening techniques
- Lack of high-throughput methods
- Failure to detect monoclonality
- Slow and resource-intensive processes
- Require multiple cellular screening assays
Technology of
Cell Line Development
The ARRALYZE CellShepherd® is the preferred platform for accelerating cell line development. ARRALYZE nanowell technology makes it ideal for culturing just a few or even a single cell.
By using nanowells, ARRALYZE accommodates thousands of wells in a small footprint, enabling experiments that would otherwise require hundreds of multi-well plates. This significantly reduces the time and resources required for cell line development. In addition, ARRALYZE isolates the desired cell live from the well and transfers it to standard multiwell plates for subsequent expansion or analysis.
1. Transfection
2. Apply selection pressure
3. Expansion
4. Individualization of cells
5. Cell dispensing
6. Ensure monoclonality
7. Hit picking and cell recovering
8. Isolation
9. Expansion
Key Features for
Cell Line Development
Using the ARRALYZE CellShepherd® for cell line development has several advantages:
High Throughput Screening
The ability to screen thousands of cells in a single run using advanced nanowell technology is a significant advance over conventional microtiter plates.
Gentle Dispensing
Dispense with the automated system in glass nanowell arrays. The system effortlessly processes single cells, cell populations, and particles, ensuring higher immobilization than manual pipetting with comparably high cell viability.
Live Imaging
The nanowells enable the culturing of individual cells. They can be imaged at any time during the experiments using standard microscope technology. This enables precise kinetic data collection.
Clonal Isolation
Precise isolation of clones while maintaining high viability using a non-destructive process that preserves cellular and clonal integrity
Proof of Monoclonality
High certainty of monoclonality with dispensing, single cell imaging, live cell tracking and tracing throughout the experimentation.
Cellular Analytics
The integrated cellular AI analysis tool accelerates the analysis of cell line development by analyzing thousands of images to gain valuable insights.
Sterile and Controlled Environment
The low contamination risk during analysis and physiological-like conditions ensure high viability of sensitive cell lines.
Applications of Cell Line Development
- Production of Biologics
- Monoclonal Antibodies
- Knock-In/Knock-Out Cell Lines for Research Purposes
- Cultivated Meat
- Production of Immortalized Cell Lines
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