Cell and Gene Therapy

Cell and gene therapy has become an indispensable tool to revolutionize the treatment of various diseases for which there are currently no effective treatment options. Cell and gene therapy involves transplanting newly created or genetically modified cells into the body to prevent or treat a disease.
The FDA has already approved six CAR-T cell therapies by the end of 2023, indicating significant progress in this area. Although currently approved CAR-T cell therapies focus only on tumors of the blood, ongoing clinical trials show initial success in clinical studies that these treatments will soon be extended to treat solid cancers. Screening cell populations and identifying outstanding cells remains a challenge.

Why Arralyze for Cell and Gene Therapy?

ARRALYZE CellShepherd^® is a comprehensive platform for research and development in cell-based therapies, offering features such as dosing, imaging, isolation, and analysis of individual cells. By identifying outliers in large cell populations, it enables a deeper understanding of what makes one NK or T cell more effective than others. It also facilitates the study of immune cell stimulation and helps uncover the mechanisms by which tumors evade the immune system.

Our goal is to fully utilize cell therapies' revolutionary possibilities to help people around the world suffering from serious diseases such as cancer, autoimmune diseases, and rare diseases. CellShepherd^® has exceptional capabilities that enhance our understanding of disease biology and drive advances in gene editing, biologics, and cell engineering.

Technology of Cell
and Gene Therapy

The ARRALYZE CellShepherd^® provides a comprehensive solution for time-resolved single cell analysis, with features like dispensing, imaging, and cell isolation. It offers a controlled environment to secure cell viability and physiologically relevant conditions.

The CellShepherd^® platform makes the behavior of individual cells visible and provides in-depth information that cannot be achieved with bulk analyses. Our platform offers real-time monitoring of cells in a fully controlled environment, allowing us to detect changes and immediate responses in cellular therapy. This continuous observation enhances the accuracy of cell therapy and imaging, which is not possible with endpoint measurements that only provide a snapshot at a single time point.

With real-time monitoring and single cell analysis, CellShepherd^® significantly improves the accuracy and efficacy of cell therapy experiments. Thanks to our innovative laser-induced deep etching (LIDE) technology, the glass arrays feature defect-free, high-aspect-ratio wells that can accommodate hundreds of cells in a compact space.

The CellShepherd^® cell analyzer facilitates experiments such as cell culture and imaging analyses that would otherwise require hundreds to thousands of multiwell plates, speeding up the identification of cells suitable for cellular therapies.

Activated tumor cells (unstained) attack and kill live target cells (stained red). The dead cells are detected using SytoxGreen in the medium, highlighting the effectiveness of the cell interaction.

CellShepherd^® also enables the study of large cell populations in small volumes in an inert environment. Even more remarkable is the ability to study cell-cell interactions in real-time and uncover individual characteristics at the single cell level. The techniques used are affordable and accessible to all researchers, as all tools for single cell analysis are combined in a single platform.

Key Features for Cell
and Gene Therapy

Using the ARRALYZE CellShepherd^® for cell and gene therapy has several advantages:

High Throughput Screening

The ability to screen thousands of cells in a single run using advanced nanowell technology is a significant advance over conventional microtiter plates.

Single Cell Dispensing

The nanowells enable the dispensing of individual cells, which can be imaged at any time during the experiments using standard microscope technology. This enables precise deposition, high encapsulation efficiency, and easy recovery.

Live Imaging

Real-time monitoring of immune cells killing tumor and other target cells in the nanowell arrays.

Clonal Isolation

Precise isolation of immune cell clones that have cytotoxic properties while maintaining viability using a non-destructive process that preserves cellular and clonal integrity.

Monoclonality

High monoclonality with live tracking and tracing of single cell imaging and dispensing throughout the experimentation.

Gentle dispensation

Dispense with the automated system in glass nanowell arrays. The system effortlessly processes single cells, cell populations, and particles, ensuring high cell viability comparable to manual pipetting.

Cellular Analytics

The integrated cellular AI analysis tool accelerates the analysis of cell line development by analyzing thousands of images to gain valuable insights.

CellSheperd Brochure (pdf - 379 KB)

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